Journal: Scientific Reports

Article Title: Exosomes exert cardioprotection in dystrophin-deficient cardiomyocytes via ERK1/2-p38/MAPK signaling

doi: 10.1038/s41598-018-34879-6

Figure Lengend Snippet: Characterization of isolated exosomes. ( a ) Electron microscopy images reveal WT- and Dys-iCM secreted exosomes display traditional cuplike morphology and are approximately 50 nm. ( b ) NTA of isolated exosomes reveals a range of sizes in particles averaging 148 nm (WT-exo) and 187 (Dys-exo). ( c) Quantitation of NTA results. ( d) Exosomes exhibit exosomal markers CD63 and CD81 as shown by flow cytometry. ( e ) Exo-Check protein array analysis reveals WT- and Dys-exo display exosome protein markers. ( f ) Cardiomyocytes are labeled with NCX1-eGFP and exosomes are stained with PKH26 (red). Exosomes are seen to be taken up at 2 hours.

Article Snippet: Exosomes isolated from a normal human adult dermal fibroblast cell line (Lonza, Basel, Switzerland) were used as an inert control.

Techniques: Isolation, Electron Microscopy, Quantitation Assay, Flow Cytometry, Protein Array, Labeling, Staining

Journal: Scientific Reports

Article Title: Exosomes exert cardioprotection in dystrophin-deficient cardiomyocytes via ERK1/2-p38/MAPK signaling

doi: 10.1038/s41598-018-34879-6

Figure Lengend Snippet: Exosomes protect against stress-induced cell injury in Dys-iCMs. Exposure to WT- and Dys-exos prior to stress ( a , b ) decreased ROS levels. Dermal fibroblast exos did not reduce stress induced ROS levels in Dys1-iCMs as significantly as iCM-derived exos. n = 3/group, *p < 0.05 vehicle stress vs. vehicle no stress, # p < 0.05 exosome exposure vs. vehicle stress.

Article Snippet: Exosomes isolated from a normal human adult dermal fibroblast cell line (Lonza, Basel, Switzerland) were used as an inert control.

Techniques: Derivative Assay